[Development and evaluation of whole-genome DNA microarray of Vibrio parahaemolyticus].

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  • Author(s): Han H;Han H; Zeng X; Yin S; Yang R; Liu X; Zhou D
  • Source:
    Wei sheng wu xue bao = Acta microbiologica Sinica [Wei Sheng Wu Xue Bao] 2010 Apr; Vol. 50 (4), pp. 548-53.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    Chinese
  • Additional Information
    • Source:
      Publisher: Science Press Country of Publication: China NLM ID: 21610860R Publication Model: Print Cited Medium: Print ISSN: 0001-6209 (Print) Linking ISSN: 00016209 NLM ISO Abbreviation: Wei Sheng Wu Xue Bao Subsets: MEDLINE
    • Publication Information:
      Publication: Beijing : Science Press
      Original Publication: [Beijing, 1953?]-
    • Subject Terms:
      Bacterial Typing Techniques*; DNA, Bacterial/*analysis ; Oligonucleotide Array Sequence Analysis/*methods ; Sequence Analysis, DNA/*methods ; Vibrio parahaemolyticus/*genetics; Gene Expression Profiling/methods ; Vibrio parahaemolyticus/physiology
    • Abstract:
      Objective: We developed and evaluated a whole-genome DNA microarray of Vibrio parahaemolyticus.
      Methods: Based on the genomic sequences of V. parahaemolyticus, we chose a total number of 4770 genes, amplified them by PCR with specific primers, purified the PCR products and printed them onto glass slides. We performed two sets of hybridizations by the method of two-fluorescence comparative hybridization to evaluate the microarray quality, followed by PCR method to validate parts of microarray results.
      Results: Microarray hybridization results were completely consistent with theory expectations and PCR verification results.
      Conclusion: We successfully developed a batch of good quality whole-genome DNA microarrays of V. parahaemolyticus, built up a method of microarray-based comparative genomic hybridization of V. parahaemolyticus and a set of microarray data analysis standard method.
    • Accession Number:
      0 (DNA, Bacterial)
    • Publication Date:
      Date Created: 20100622 Date Completed: 20100707 Latest Revision: 20201209
    • Publication Date:
      20231215
    • Accession Number:
      20560361