ىسررب ىيأسئاشو ىأهجراق هارمه هضراع لاوز ناتخرد سرا (Arabic)

Investigation and identification of fungi associated with juniper tree decline. (English)

In order to identify fungal agents associated with juniper trees in the highlands of Tarm County, samples of various tissues from affected trees were collected and subjected to cultivation and isolation using conventional methods. For species identification, both morphological and molecular methods were employed. To achieve this, DNA was extracted from 11 fungal species isolated. The internal transcribed spacer (ITS) region of their ribosomal DNA was amplified using universal primers (ITS-1 and ITS-4). Subsequently, their sequences were determined and compared to sequences available in databases to confirm the identification of species. In total, more than 150 fungal isolates were separated from diseased juniper trees over a span of three years. Based on morphological characteristics and ITS sequence analysis, the following fungal species were confirmed: Nigrospora oryzae (31 isolates), Penicillium spp. (9 isolates), Aspergillus spp. (4 isolates), Trichoderma spp. (5 isolates), Aureobasidium pullulans (33 isolates), Polyporus sp. (3 isolates), Phoma sp. (14 isolates), Phoma medicaginesis (3 isolates), Alternaria alternata (5 isolates), Alternaria sp. (9 isolates), Alternaria consortialis (6 isolates), Hormonema carpetanum (5 isolates), Hormonema sp. (9 isolates), Peyronellaea pinodella (4 isolates), Kabatiella microsticta (3 isolates), and Microsphaeropsis spp. (8 isolates). Most of these species are opportunistic pathogens, reported for the first time on juniper trees in Iran. These agents, in conjunction with host vulnerability due to infestations by phytophagous insects, drought stress, and pest attacks, accelerate the decline of the host trees. [ABSTRACT FROM AUTHOR]

روظنمدب ىياسانش لماوع ىحراق هارمه اب ناتخرد سرا رد تاعافترا ناتسرهش ،مراط ىياهدنومن زا ىاهتفاب فلتخم ناتخرد راميب هيبهت و اب هداغتسا زا ىاهرور لومعم تبسن هب تشك و ىزاسادج اهنآ مادقا .ديدرك ىارب ىياسانش ،اههنوك زا ىاهرور ىسررب ىساذشتخير و ىياسانش ىلوكلوم هداغتسا .دش ىارب نيا ،روظنم نمض جارختسا DNAلك زا دادعت 11 هنوك زا ىاهجراق ىزاسادج ،هدش هيحان زادناهلصاف ىلخاد DNA ىموزوبيار اهنآ اب هداغتسا زا ىاهرميارب ىمومع)1-ITS و 4-ITS(ريثكت و دعب اب ىبايروت اهنآ و هسياقم اب ىاهىلاوت دوجوم رد ىاهدناب ىتاعلاطا ىياسانش اههنوك ماجنا .دش عومجمرد رتيب زا 105 هيادج ىحراق رد تدم هس لاس زا ىاهدنومن هدولآ ناتخرد سرا ىزاسادج دش هك رب ساسا ىاهىكزيو ىرهاظ و ىلاوت ىباي هيجان ITS ىاههيادج ،ىحراق قلعت اهنآ هب ىاههنوك ريز دييأت :ديدرك 01 (Nigrospora oryzae ،(هيادج .0(Penicillium spp ،(هيادج .0(Aspergillus spp ،(هيادج 0(Trichoderma spp. ،(هيادج 00(Aureobasidium pullulans ،(هيادج .0(Polyporus sp ،(هيادج .10(Phoma sp ،(هيادج Phoma 0١،(نجدج Alternaria alternata 0١ ،هذدج .(Alternaria sp 0١ ،هذدج Iternaria consortia! is ١ ،هذدج Hormonema carpetanum 0١ ،هذدج .(Hormonema sp 0١ ،اسدج Peyronehaeapinodeha 0١ ،هذدج Kabatiella 0(microsticta (هيادج و.2(Microsphaeropsis spp .(هيادج رتشيب اههنوك تيفورباس اي لماوع ىازىراميب بلطذسرف دنتسه هك نيلوا راب رد ناريا زا ىور سرا شرازك .دذوشىم نيا لداوع نامزمه اب فعض نابزيم رد رث'ا لاتبا هب هايك لاكنا ،كشاوراد شنت ىكشخ و تافآ اب هلمح هب اهنآ ثعاب عيرست لاوز نابزيبم .دذوشىم. [ABSTRACT FROM AUTHOR]

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