Isolation of a candidate human hematopoietic stem-cell population.

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  • Author(s): Baum CM;Baum CM; Weissman IL; Tsukamoto AS; Buckle AM; Peault B
  • Source:
    Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 1992 Apr 01; Vol. 89 (7), pp. 2804-8.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print Cited Medium: Print ISSN: 0027-8424 (Print) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE
    • Publication Information:
      Original Publication: Washington, DC : National Academy of Sciences
    • Subject Terms:
      Bone Marrow Cells* ; Hematopoiesis*; Hematopoietic Stem Cells/*cytology; Animals ; Antigens, CD/analysis ; Antigens, CD34 ; Antigens, Surface/analysis ; Cell Differentiation ; Cell Separation ; Cells, Cultured ; Colony-Forming Units Assay ; Flow Cytometry ; Humans ; In Vitro Techniques ; Lymphocytes/cytology ; Membrane Glycoproteins/analysis ; Mice ; Mice, SCID ; Thy-1 Antigens ; Thymus Gland/cytology
    • Abstract:
      We have identified a rare (0.05-0.1%) subset of human fetal bone marrow cells that contains multipotent hematopoietic precursors. The population of human precursor cells that express Thy-1 and CD34 but no known lineage markers is enriched for clonogenic activity that establishes long-term, multilineage (myelomonocytic and B lymphoid) cultures on mouse marrow stromal lines. Further, the Thy-1+CD34+ subset that takes up little of the fluorescent mitochondrial dye rhodamine 123 contains virtually all the cells that establish long-term cultures. In human fetal thymus transplanted into SCID (severe combined immunodeficiency) mice, Thy-1+CD34+ fetal bone marrow cells differentiate into T lymphocytes. In two of nine cases, allogeneic Thy-1+CD34+ cells could engraft intact human fetal bone marrow grown in SCID mice, resulting in donor-derived myeloid and B cells. By extrapolation, the rare human Thy-1+Lin-CD34+ cell population contains pluripotent hematopoietic progenitors; we propose that it is highly enriched for candidate hematopoietic stem cells.
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    • Grant Information:
      CD49605 United States CD ODCDC CDC HHS
    • Accession Number:
      0 (Antigens, CD)
      0 (Antigens, CD34)
      0 (Antigens, Surface)
      0 (Membrane Glycoproteins)
      0 (Thy-1 Antigens)
    • Publication Date:
      Date Created: 19920401 Date Completed: 19920506 Latest Revision: 20220321
    • Publication Date:
      20221213
    • Accession Number:
      PMC48751
    • Accession Number:
      10.1073/pnas.89.7.2804
    • Accession Number:
      1372992