Enhanced production of microbial metabolites in the presence of dimethyl sulfoxide.

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  • Author(s): Chen G;Chen G; Wang GY; Li X; Waters B; Davies J
  • Source:
    The Journal of antibiotics [J Antibiot (Tokyo)] 2000 Oct; Vol. 53 (10), pp. 1145-53.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Nature Publishing Group Country of Publication: England NLM ID: 0151115 Publication Model: Print Cited Medium: Print ISSN: 0021-8820 (Print) Linking ISSN: 00218820 NLM ISO Abbreviation: J Antibiot (Tokyo) Subsets: MEDLINE
    • Publication Information:
      Publication: 2009- : London : Nature Publishing Group
      Original Publication: Tokyo, Japan Antibiotics Research Assn.
    • Subject Terms:
      Anti-Bacterial Agents/*biosynthesis ; Bacillus/*drug effects ; Bacillus/*metabolism ; Dimethyl Sulfoxide/*pharmacology ; Naphthacenes/*metabolism ; Streptomyces/*drug effects ; Streptomyces/*metabolism; Bacillus/growth & development ; Chloramphenicol/biosynthesis ; Culture Media ; Organic Chemicals/pharmacology ; Plasmids ; Streptomyces/genetics ; Streptomyces/growth & development ; Sulfones/pharmacology ; Thiostrepton/biosynthesis
    • Abstract:
      Bacterial strains grown in the presence of low concentrations of dimethyl sulfoxide (DMSO) exhibit significant qualitative and quantitative alterations in the production of secondary metabolites. This effect was confirmed for a variety of biosynthetic families, including chloramphenicol (chorismate), thiostrepton (peptide) and tetracenomycin (polyketide), and for natural and recombinant strains of streptomycetes; a similar effect was seen with antibiotic-producing bacilli such as B. circulans. Increase in antibiotic production was not the result of a change in the growth rate of these organisms, since yields of biomass were similar in media with and without DMSO (up to 3%). We suggest that the addition of compounds such as DMSO provides a means of examining the full biosynthetic potential of microbes and might be used to promote secondary metabolite production. The mode of action of DMSO is not known, but in the cases studied it may act at the level of translation.
    • Accession Number:
      0 (Anti-Bacterial Agents)
      0 (Culture Media)
      0 (Naphthacenes)
      0 (Organic Chemicals)
      0 (Sulfones)
      146864-75-7 (5838 DNI)
      66974FR9Q1 (Chloramphenicol)
      9H4PO4Z4FT (dimethyl sulfone)
      HR4S203Y18 (Thiostrepton)
      YOW8V9698H (Dimethyl Sulfoxide)
    • Publication Date:
      Date Created: 20010102 Date Completed: 20010118 Latest Revision: 20190723
    • Publication Date:
      20240829
    • Accession Number:
      10.7164/antibiotics.53.1145
    • Accession Number:
      11132960